Journal: BMC Genomics

Article Title: ChIP on Chip: surprising results are often artifacts

doi: 10.1186/1471-2164-11-414

Figure Lengend Snippet: Detection of critical steps in the ChIP-Chip protocol . A Ratios of ChIP DNA purified with spin column versus column free purified DNA detected by qPCR for the indicated gene regions. B qPCR ratios of crosslinked-reversed versus non crosslinked DNA for indicated gene regions. C Crosslinked-reversed versus non crosslinked DNA as shown in B was differentially labeled and hybridized to a microarray. Log 2 ratios are shown in blue (only values below -0.5) in comparison to the SeqA ChIP signal (from Fig. 2).

Article Snippet: We thank Franz Narberhaus (Bochum) for the σ 32 antiserum and Douglas Hurd (Oxford Gene Technology) for instruction in DNA labeling and microarray hybridization.

Techniques: ChIP-chip, Purification, Labeling, Microarray, Comparison